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PLASMIDS:

We are distributing the CRY/CIB plasmids on Addgene.

Additional sequences are available for downloading below. Please contact chandra.tucker@ ucdenver.edu for more information about plasmids.

Kennedy et al., (2010) Rapid blue light induction of protein interactions in living cells. Nature Methods 7, 973-975.

name of construct text sequence Ape format resistance
CRY2-mCh CRY2(deltaNLS)-mCh seq Kan

CRY2PHR-mCh

CRY2PHR-mCh seq Kan
CIBN-pmGFP CIBN(deltaNLS)-pmGFP seq Kan
CRY2-CreN mCh-IRES-CRY2-CreN seq Kan
CIBN-CreC mCh-IRES-CIBN-CreC seq Kan
GAL4BD-CRY2 pDBTrp-CRY2 seq Kan
GAL4BD-CRY2PHR pDBTrp-CRY2PHR seq Kan
GAL4AD-CIB1 pGADT7rec-CIB1 seq Amp
GAL4AD-CIBN pGADT7rec-CIBN seq Amp

Taslimi et al., (2014) An optimized optogenetic clustering tool for probing protein interaction and function. Nature Commun. 5: 4925

name of construct text sequence Ape format resistance
CRY2olig-mCh CRY2olig-mCh seq Kan

CRY2oligFL(deltaNLS)-mCh

CRY2oligFL-mCh seq Kan
CRY2olig-mCh-CLC CRY2olig-mCh-CLC seq Kan

Taslimi et al., (2016) Optimized second generation CRY2/CIB dimerizers and photoactivatable Cre recombinase. Nature Chem. Biol. 12, 425-430.

name of construct text sequence Ape format resistance
CIB1-CreC (mChN1 backbone) CIB1-CreC seq Kan

CRY2(L348F)-CreN (mCh-IRES backbone)

CRY2(L348F)-CreN seq Kan
pDBTrp-CRY2(535) Gal4BD-CRY2(535) seq Kan
CIB81-pmGFP CIB81-pmGFP seq Kan
CRY2(535) L348F-mCh CRY2(535)L348F-mCh seq Kan
CRY2PHR(W349R)-mCh CRY2PHR(W349R)-mCh seq Kan

Liu et al., (2019) A photoactivatable botulinum neurotoxin for inducible control of neurotransmission. Neuron 101, 863-875.

Construct name Addgene link Ape format resistance
pQL47 EGFP-VAMP-GST   pQL47 Kan

pQL193 BoNT(1-146)-iLID(V416I)

  pQL193 Kan
pQL185 SspB(milli)-BoNT(147-441,Y365A)   pQL185 Kan
pQL261 pAAV-hSYN-mCh-IRES-SspB(A58V/R73Q)-BoNT/B(147-441,Y365A)   pQL261 Kan
pQL262 pAAV-hSYN-mCh-IRES-BoNT/B(1-146)-iLID(V416I)   pQL262 Kan
pQL269 pAAV-hSYN-mCh-IRES-SspB(R73Q)-BoNT/B(147-441,Y365A)   pQL269 Kan
pQL281 pAAV-hSYN-SYP-GFP-BoNT/B(1-146)-iLID(V416I)   pQL281 Kan

SOFTWARE:

LEDs software, described in Hernandez-Candia et al., Advances in optogenetic regulation of gene expression in mammalian cells using cryptochrome 2 (CRY2).

Software for programming LED modules, described in "Tools for Controlling Protein Interactions Using Light', Current Protocols in Cell Biology 17.16.1-17.16.20.