Hernandez-Candia, C. N., Pearce, S., and Tucker, C.L. A modular tool to query and inducibly disrupt biomolecular condensates. (2021) Nat. Commun.


Hernandez-Candia, C. N. and Tucker, C.L. Optogenetic control of gene expression using cryptochrome 2 and a light-activated degron. Methods in Molecular Biology, doi: 10.1007/978-1-0716-0755-8_10.

Cleveland, J. and Tucker, C.L. Photo-SNAP-tag, a light-regulated chemical labeling system. (2020) ACS Chemical Biology 15, 2212-2220.

Pearce, S. and Tucker, C.L. Dual Systems for Enhancing Control of Protein Activity through Induced Dimerization Approaches, Adv. Biology.


Meador, K.M., Wysoczynski, C.L. Norris, A.J., Aoto, J., Bruchas, M.R., and *Tucker, C.L. Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent. (2019) Nucleic Acids Res. gkz585

^Liu, Q., ^Sinnen, B.L., Boxer, E.E., Buchta, W.C., Schneider, M.W., Gibson, E.S., Wysoczynski, C.L., Gottschalk, A., Aoto, J., *Tucker, C.L., and *Kennedy, M.J. A photoactivatable botulinum neurotoxin for inducible control of neurotransmission. (2019) Neuron 101, 863-875.
^Co-first author *Co-corresponding author. (link to article)

Spiltoir, J.I. and Tucker, C.L. Photodimerization systems for regulating protein-protein interactions with light. (2019) Curr. Opin. Struct. Biol. 57, 1-8. (link to article)

Hernandez-Candia, C. N., Wysoczynski, C.L., and Tucker, C.L., Advances in optogenetic regulation of mammalian cells using cryptochrome 2 (CRY2). (2019) Methods, (link to article)


Cook, S.G., Bourke, A.M., O'Leary, H., Zaegel, V., Lasda, E., Minze-Berge, J., Quillinan, N., Tucker, C.L., Coultrap, S.J., Herson, P.S., and Bayer, K.U. Analysis of the CaMKIIalpha and beta splice-variant distribution among brain regions reveals isoform-specific differences in holoenzyme formation. (2018) Scientific Reports 8, 5448. (link to article)

Locke, C., Machida, K., Tucker, C.L., Wu, Y., and Yu, J. Optogenetic activation of EphB2 receptor in dendrites induced actin polymerization by activating Arg kinase. (2017) Biol. Open 6, 1820-1830.


Pathak, G., Spiltoir, J.I., Hoglund, C., Polstein, L.R., Heine-Koskinen, S., Gersbach, C.A., Rossi, J., and Tucker, C.L. (2017) Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res. doi 10.1093/nar/gkx260. (link to article)

Liu, Q., and Tucker, C.L. Engineering genetically-encoded tools for optogenetic control of protein activity. (2017) Current Opinion in Chem. Biol.40, 17-23. (link to article)


Taslimi, A., Zoltowski, B., Miranda, J.G., Pathak, G., Hughes, R.M., and Tucker, C.L. (2016) Optimized second generation CRY2/CIB dimerizers and photoactivatable Cre recombinase. Nat. Chem. Biol. 12, 425-430. (link to article) (view-only PDF)

Spiltoir, J.I., Strickland, D., Glotzer, M., and Tucker, C.L. (2016) Optical control of peroxisomal trafficking. ACS Synthetic Biology 5, 554-560.(Link to article)


Schindler, SE., McCall, J.G., Yan, P., Hyrc, KL., Li, M., Tucker, C.L., Lee, J.M., Bruchas, M.R., Diamond, M.I. (2015) Sci. Reports 5, 13627. (link to article)


Pathak, G.P., Strickland, D., Vrana, J.D., and Tucker, C.L. (2014) Benchmarking of optical dimerizer systems. ACS Synthetic Biology, 3, 832–838. (link to article)

Taslimi, A., Vrana, J.D., Chen, D., Borinskaya, S., Mayer, B., Kennedy, M.J., and Tucker, C.L. An optimized optogenetic clustering tool for probing protein interaction and function. (2014) Nature Commun. 5: 4925. **Highlighted in Nature Methods, Nov 2014 issue** (link to article)

Tucker, C.L., Vrana, J.D., and Kennedy, M.J. (2014) Tools for controlling protein interactions with light. Curr. Protoc. Cell Biol. 64, 17.16.1-17.16.20.

Hansen, M.G., Fregoso, V., Vrana, J.D., Tucker, C.L., and Niswander, L.A. Peripheral nervous system defects in a mouse model for peroxisomal biogenesis disorders. (2014) Dev. Biol.395, 84-95

Lage, M.D., Pittman, A.M., Roncador, A., Cellini, B., and Tucker, C.L. (2014) Allele-specific characterization of alanine:glyoxylate aminotransferase variants associated with primary hyperoxaluria. PLOS ONE 9, e94338.


Pathak, G.P., Vrana, J.D., and Tucker, C.L. (2013) Optogenetic control of cell function using engineered photoreceptors. Biol Cell 105, 59-72.


Pittman, A.M., Lage, M.D., Poltoratsky, V., Vrana, J., Paiardini, A., Roncador, A., Cellini, B., Hughes, R.M., and Tucker, C.L. (2012) Rapid profiling of disease alleles using a tunable reporter of protein misfolding. Genetics 192, 831-842. **Highlighted in Nature Reviews Genetics **

Hughes, R.M., Bolger, S., Tapadia, H., and Tucker, C.L. (2012) Light-mediated control of DNA transcription in yeast. Methods. 58, 385-391.

Hughes, R.M., Vrana, J.D., Song, J., and Tucker, C.L. (2012) A light-dependent, dark-promoted interaction of Arabidopsis cryptochrome 1 and phytochrome B. J. Biol. Chem. 287, 22165-22172. *Paper of the Week*  **Awarded best plant biology paper in JBC in 2012 **

Tucker, C.L. (2012) Manipulating cellular processes using optical control of protein-protein interactions. Prog. Brain Res. 196, 95-117.

Pajerowska-Mukhtar, K.M., Wang, W., Tada, Y., Oka, N., Tucker, C.L., Fonseca, J.P., and Dong, X. (2012) The HSF-like transcription factor TBF1 is a major molecular switch for plant growth-to-defense transition. Curr Biol. 22, 103-112.


Kennedy, M.J., Hughes, R.M, Peteya, L.A., Schwartz, J.W., Ehlers, M.D., and Tucker, C.L. Rapid blue light induction of protein interactions in living cells. (2010) Nature Meth 7, 973-975.

West, G.M., Tucker, C.L., Xu, T., Park, S.K., Han, X., Yates, J.R., and Fitzgerald, M.C. (2010) Quantitative proteomics approach for identifying protein-drug interactions in complex mixtures using protein stability measurements. Proc. Natl. Acad. Sci. U.S.A. 107, 9078-9082.

Hopper, E. D., Pittman, A.M.C., Tucker, C. L., Campa, M. J., Patz, E. F., and Fitzgerald, M. C. (2009) Hydrogen/deuterium exchange- and protease digestion-based screening assay for protein-ligand binding detection. Anal. Chem. 81, 6860-6867.

Tucker, C. L., Peteya, L.. Pittman, M. C., and Zhong, J. (2009) A genetic test for two-hybrid bait competency using RanBPM. Genetics 182, 1377-1379.

Hopper, E. D., Pittman, A.M.C., Fitzgerald, M.C., and Tucker, C. L. (2008) In vivo and in vitro examination of stability of primary hyperoxaluria-associated human alanine:glyoxylate aminotransferase. J. Biol. Chem. 283, 30493-30502.

Graduate / Postdoctoral

Tucker, C. L. and Fields, S. (2004) Quantitative genome-wide analysis of yeast deletion strain sensitivities to oxidative and chemical stress. Comp. & Funct. Genomics 5, 216-224.

Tucker, C. L. , Ramamurthy, V., Pina, A., Loyer, M., Dharmaraj, S., Li, Y., Maumenee, I., Hurley, J. B., and Koenekoop, R. K. (2004) Functional analyses of mutant recessive GUCY2D alleles identified in Leber congenital amaurosis patients. Mol. Vision 10, 297-303.

Tucker, C. L. (2002) High-throughput cell-based assays in yeast. Drug Discovery Today 7 (Suppl.), S125-S130. pdf.

Tucker, C. L. and Fields, S. (2001) Engineering a yeast sensor of ligand binding. Nature Biotechnology 19, 1042-1046. ** highlighted in: Nature Biotechnology 19, 1022-1023; Nature Reviews Drug Discovery 1, 6-7; Analytical Chemistry 74, 13A ** pdf.

Tucker, C. L. , Gera, J. F. and Uetz, P. (2001) Towards an understanding of complex protein networks. Trends Cell Biol. 11, 102-106.

Ramamurthy, V., Tucker, C., Wilkie, S.E., Daggett, V., Hunt, D.M., and Hurley, J.B. (2001) Interactions within the coiled-coil domain of RetGC-1 guanylyl cyclase are optimized for regulation rather than high affinity. J. Biol. Chem. 276, 26218-26229.

Tucker, C. L. , Woodcock, S. C., Kelsell, R. E., Hunt, D. M., and Hurley, J. B. (1999) Biochemical analysis of a dimerization domain mutation in RetGC-1 associated with dominant cone-rod dystrophy. Proc. Natl. Acad. Sci. U.S.A. 96, 9039-9044.

Tucker, C. L ., Hurley, J. H., Miller, T. R., and Hurley, J. B. (1998) Two amino acids convert a guanylyl cyclase, RetGC-1, into an adenylyl cyclase. Proc. Natl. Acad. Sci. U.S.A. 95, 5993-5997.

Tucker, C. L., Laura, R. P., and Hurley, J. B.  (1997)  Domain-specific stabilization of photoreceptor membrane guanylyl cyclase by adenine nucleotides and guanylyl cyclase activating proteins (GCAPs). Biochemistry 36, 11995-2000.