PLASMIDS:
We are distributing the CRY/CIB plasmids on Addgene.
Additional sequences are available for downloading below. Please contact chandra.tucker@ ucdenver.edu for more information about plasmids.
Kennedy et al., (2010) Rapid blue light induction of protein interactions in living cells. Nature Methods 7, 973-975.
| name of construct | text sequence | Ape format | resistance |
| CRY2-mCh | CRY2(deltaNLS)-mCh | seq | Kan |
CRY2PHR-mCh |
CRY2PHR-mCh | seq | Kan |
| CIBN-pmGFP | CIBN(deltaNLS)-pmGFP | seq | Kan |
| CRY2-CreN | mCh-IRES-CRY2-CreN | seq | Kan |
| CIBN-CreC | mCh-IRES-CIBN-CreC | seq | Kan |
| GAL4BD-CRY2 | pDBTrp-CRY2 | seq | Kan |
| GAL4BD-CRY2PHR | pDBTrp-CRY2PHR | seq | Kan |
| GAL4AD-CIB1 | pGADT7rec-CIB1 | seq | Amp |
| GAL4AD-CIBN | pGADT7rec-CIBN | seq | Amp |
Taslimi et al., (2014) An optimized optogenetic clustering tool for probing protein interaction and function. Nature Commun. 5: 4925
| name of construct | text sequence | Ape format | resistance |
| CRY2olig-mCh | CRY2olig-mCh | seq | Kan |
CRY2oligFL(deltaNLS)-mCh |
CRY2oligFL-mCh | seq | Kan |
| CRY2olig-mCh-CLC | CRY2olig-mCh-CLC | seq | Kan |
Taslimi et al., (2016) Optimized second generation CRY2/CIB dimerizers and photoactivatable Cre recombinase. Nature Chem. Biol. 12, 425-430.
| name of construct | text sequence | Ape format | resistance |
| CIB1-CreC (mChN1 backbone) | CIB1-CreC | seq | Kan |
CRY2(L348F)-CreN (mCh-IRES backbone) |
CRY2(L348F)-CreN | seq | Kan |
| pDBTrp-CRY2(535) | Gal4BD-CRY2(535) | seq | Kan |
| CIB81-pmGFP | CIB81-pmGFP | seq | Kan |
| CRY2(535) L348F-mCh | CRY2(535)L348F-mCh | seq | Kan |
| CRY2PHR(W349R)-mCh | CRY2PHR(W349R)-mCh | seq | Kan |
Liu et al., (2019) A photoactivatable botulinum neurotoxin for inducible control of neurotransmission. Neuron 101, 863-875.
| Construct name | Addgene link | Ape format | resistance |
| pQL47 EGFP-VAMP-GST | pQL47 | Kan | |
pQL193 BoNT(1-146)-iLID(V416I) |
pQL193 | Kan | |
| pQL185 SspB(milli)-BoNT(147-441,Y365A) | pQL185 | Kan | |
| pQL261 pAAV-hSYN-mCh-IRES-SspB(A58V/R73Q)-BoNT/B(147-441,Y365A) | pQL261 | Kan | |
| pQL262 pAAV-hSYN-mCh-IRES-BoNT/B(1-146)-iLID(V416I) | pQL262 | Kan | |
| pQL269 pAAV-hSYN-mCh-IRES-SspB(R73Q)-BoNT/B(147-441,Y365A) | pQL269 | Kan | |
| pQL281 pAAV-hSYN-SYP-GFP-BoNT/B(1-146)-iLID(V416I) | pQL281 | Kan |
SOFTWARE:
LEDs software, described in Hernandez-Candia et al., Advances in optogenetic regulation of gene expression in mammalian cells using cryptochrome 2 (CRY2).
Software for programming LED modules, described in "Tools for Controlling Protein Interactions Using Light', Current Protocols in Cell Biology 17.16.1-17.16.20.